AICF offers access to a variety of microscopes for imaging of live and fixed cells/tissues. This page offers a brief introduction to the equipment in our facility; hopefully we are able to meet the needs of your academic or industrial research requirements. Please jump to the Administration page to review full details on training program, user fees, and reserving time on one of our microscopes.
1. Zeiss LSM 510 Laser Scanning Confocal Microscope
- Zeiss AxioVert200M inverted platform
- 10x, 20x, 40x water, 40x oil, 63x oil
- Excitation - 458, 477, 488, 514, 561, 633
- Emission - Meta Detector spectral range (361.8-704.2)
- Filter Cubes – DAPI, CFP, GFP, Rhodamine, DIC
- Environmental Control: heated stage, incubation chamber, CO2 supply
- Sample Format - slides, glass-bottom dish, microwell plate
- Zen Operating System
2. Zeiss Spinning Disc Cell Observer Confocal Microscope
- Zeiss AxioObserver Z1 inverted platform
- Objectives - 10x, 20x, 40x oil, 63x oil, 100x oil
- Excitation - 405, 458, 488, 514, 561, 639
- Emission - 450/50, 525/50, 535/50, 629/62, 690/50
- Environmental Control: heated stage, incubation chamber, CO2 supply
- Sample Format - slides, glass-bottom dish, microwell plate
- Filter cubes -- DAPI, GFP, Rhodamine, DIC, 2-color (GFP+RFP), 80/20 splitter
- Cameras -- Photometrics Evolve, Zeiss MRm
- Zen Operating System
3. Zeiss-Perkin-Elmer UltraView Spinning Disc Confocal Microscope
- Zeiss AxioExaminer Z1 upright platform
- Objectives - 10x, 20x, 40x dipping, 63x oil
- Excitation - 488, 568, 647
- Emission - 535, 629, 690
- Temperature controlled stage
- Filter Cubes - DAPI, GFP, Rhodamine, DIC
- Cameras - Hamamatsu OrcaER, Zeiss MRm
- Volocity Operating System
4. Zeiss Vivatome Structured Illumination Microscope
- Zeiss AxioVert200M inverted platform
- Objectives - 10x, 20x, 40x dry, 40x oil, 63x oil, 100x oil
- Excitation/Emission Filter Cubes - DAPI, GFP, Rhodamine (Wide field), Texas Red (Vivatome)
- Cameras - Zeiss MRm
- Axiovision Operating System
5. Zeiss SteREO Discovery V12 Stereo Microscope
- Epifluorescence Capable
- Excitation – 488, 590
- Filter Cubes – GFP, mCherry
- Camera – Hamamatsu OrcaER
- Micromanager, ImageJ
6. ThermoFisher-FEI Tecnai12 Bio-twin TEM
- Camera – Gatan OneView 4k-by-4k CMOS camera
- DigitalMicrograph Software
- Goniometer Stage for tilt imaging
- Digital Montage Software of montaging and stitching images
EM Suite Support Equipment
- Ultramicrotome – Leica EM-UC7
- Leica-Reichart Ultracut E
- LKB knifemakers
- Pelco easiGlow – Glow Discharge Apparatus
- Complete wet lab for tissue processing, embedding.