601D Life Sciences Center
Understanding the mechanisms that govern neuronal wiring is a central focus of developmental neurobiology and, importantly, will result in the identification of molecular mechanisms relevant to many disease processes. The overarching goal of our research is to better understand how molecular cues regulate neuronal morphogenesis, synapse development and refinement, which lead to the proper wiring of the nervous system that ultimately result in the execution of complex behavior in the organism.
Accordingly, our research team is interested in the following questions: 1) what are the molecules controlling neural circuit formation? 2) how are these connections maintained throughout life? and 3) what are the underlying cellular mechanisms controlling axonal guidance versus synapse formation? To address these questions, we will employ cellular, molecular, and genetic approaches to analyze both the central and peripheral arms of the mouse nervous system. We will use a combination of interdisciplinary approaches including, but not limited to, sophisticated mouse genetics (inducible knockout/knockin, CRISPR) to perform in vitro and in vivo experiments, and physiology and behavioral analysis to provide a platform to study complex neural circuit functions and how defects in salient connections may lead to the development of neurological disorders, such as autism spectrum disorder.
Recently taught courses:
2016 - present (spring): Introduction to Neuroanatomy: Structure and Function (21:120:404, Undergrad); Course Director.
2019 (fall): Topics in Biology: Neurodevelopment (26:120:616, Grad); Course Director.
2018 (fall): Cell Biology (21:120:355, Undergrad); Co-course Instructor.
B.S. in Physiological Sciences/Neuroscience, University of California, Los Angeles, 1998.
Ph.D. in Molecular, Cellular and Integrative Physiology, University of California, Los Angeles, 2003.
Danelon, V, Goldner, R, Martinez, E, Gokhman, I, Wang, K, Yaron, A, Tran, TS. (2020). Modular and distinct Plexin-A4/FARP2/Rac1 signaling controls dendrite morphogenesis. J. Neurosci. 40:5413-5430. DOI: https://doi.org/10.1523/JNEUROSCI.2730-19.2020. (Cover Article)
Assous, M, Martinez, E, Eisenberg, C, Shah, F, Kosc, A, Varghese, K, Espinoza, D, Bhimani, S, Tepper, JM, Shiflett, MW, Tran, TS. (2019). Neuropilin 2 Signaling Mediates Corticostriatal Transmission, Spine Maintenance, and Goal-Directed Learning in Mice. J Neurosci. 39:8845-8859. doi: 10.1523/JNEUROSCI.1006-19.2019.
Kung F, Wang, W, Tran, TS, Townes-Anderson, E. (2017). Sema3A Reduces Sprouting of Adult Rod Photoreceptors In Vitro. Invest Opthalmol Vis Sci. 58:4318-433`. doi: 10.1167/iovs.16-21075.
Shiflett, MW, Martinez, E, Khdour, H, Tran, TS. (2017). Functions of Neuropilins in Wiring the Nervous System and Their Role in Neurological Disorders. In: The Neuropilins: Role and Function in Health and Disease. Neufeld G, Kessler O, editors. Switzerland: Springer Nature; Chapter 8; p.125-149.
Peng, SS, Tran, TS. (2017). Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins: A Primary Culture Approach. Methods Mol Biol. 1493:209-222. doi: 10.1007/978-1-4939-6448-2_15.
Hernandez-Enriquez, B, Wu, Z, Martinez, E, Olsen, O, Kaprielian, Z, Maness, PF, Yoshida, Y, Tessier-Lavigne, M, Tran, TS. (2015). Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing. Genes Dev. 29:2617-2632. doi: 10.1101/gad.268086.115.
Martinez, E, Tran, TS. (2015). Vertebrate spinal commissural neurons: a model system for studying axon guidance beyond the midline. Wiley Interdiscip Rev Dev Biol. 4:283-297. doi: 10.1002/wdev.173.
Shiflett, MW, Gavin, M, Tran, TS. (2015). Altered hippocampal-dependent memory and motor function in neuropilin 2-deficient mice. Transl Psychiatry. 5:e521. doi: 10.1038/tp.2015.17.
Yu, S, Yehia, G, Wang, J, Stypulkowski, E, Sakamori, R, Jiang, P, Hernandez-Enriquez, B, Tran, TS, Bonder, EM, Guo, W, Gao, N. (2014). Global ablation of the mouse Rab11a gene impairs early embryogenesis and matrix metalloproteinase secretion. J Biol Chem. 289:32030-32043. doi: 10.1074/jbc.M113.538223.
Demyanenko, GP, Mohan, V, Zhang, X, Brennaman, LH, Dharbal, KE, Tran, TS, Manis, PB, Maness, PF. (2014). Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci. 34:11274-11287. doi: 10.1523/JNEUROSCI.1774-14.2014.
Mlechkovich, G, Peng, SS, Shacham, V, Martinez, E, Gokhaman, I, Minis, A, *Tran, TS, *Yaron, A. (2014). Distinct cytoplasmic domains in Plexin-A4 mediate diverse responses to semaphorin 3A in developing mammalian neurons. Sci. Signal. 7:ra24. doi: 10.1126/scisignal.2004734. (*co-corresponding authors)
*#Tran, TS, *Carlin, E, Lin, R, Martinez, E, Johnson, JE, #Kaprielian, Z. (2013). Neuropilin 2 regulates the guidance of post-crossing spinal commissural axons in a subtype-specific manner. Neural Dev. 8:15. doi: 10.1186/1749-8104-8-15. (*co-first and #co-corresponding authors)
Calderon de Anda, F, Rosario, AL, Durak, O, Tran, T, Graff, J, Meletis, K, Rei, D, Soda, T, Madabhushi, R, Ginty, DD, Kolodkin, AL, Tsai, L. (2012). Autism spectrum disorder susceptibility gene TAOK2 affects basal dendrite formation in the neocortex. Nat. Neurosci. 15: 1022-31. doi: 10.1038/nn.3141.
Becker, PM, Tran, TS, Delannoy, MJ, He, CX, Shannon, JM, McGrath-Morrow, S. (2011). Semaphorin 3A contributes to distal pulmonary epithelial cell differentiation and lung morphogenesis. PLoS One 6. doi: 10.1371/journal.pone.0027449.
Demyanenko, GP, Riday, TT, Tran, TS, Dalal, J, Darnell, EP, Brennaman, LH, Sakurai, T, Grumet, M, Philpot, BD, Maness, PF. (2011). NrCAM deletion causes topographic mistargeting of thalamocortical axons to the visual cortex and disrupts visual acuity. J. Neurosci. 31:1545-1558. doi: 10.1523/JNEUROSCI.4467-10.2011.
Tran, TS, Rubio, ME, Clem, RL, Johnson, D, Case, L, Tessier-Lavigne, M, Huganir, RL, Ginty, DD and Kolodkin, AL. (2009). Secreted semaphorins control spine distribution and morphogenesis in the postnatal CNS. Nature, 462:1065-1069. doi: 10.1038/nature08628.
Kolk, SM, Gunput, RF, Tran, TS, van den Heuvel, DMA, Prasad, AA, Hellemons, AJGM, Adolfs, Y, Ginty, DD, Kolodkin, AL, Burbach, PH, Smidt, MP, Pasterkamp, RJ. (2009). Semaphorin 3F is a bifunctional guidance cue for dopaminergic axons and controls their fasciculation, channeling, rostral growth and intracortical targeting. J. Neurosci. 29:12542-12557. doi: 10.1523/JNEUROSCI.2521-09.2009.
Wright, AG, Demyanenko, GP, Powell, A, Schachner, M, Enriquez-Barreto L, Tran, TS, Polleux, F, and Maness, PF. (2007). Close Homolog of L1 and Neuropilin 1 mediate guidance of thalamocortical axons at the ventral telencephalon. J. Neurosci. 27:13667- 13679. doi: 10.1523/JNEUROSCI.2888-07.2007.
Tran, TS, Kolodkin, AL, and Bharadwaj, R. (2007). Semaphorin regulation of cellular morphology. Ann. Rev. Cell Dev. Biol. 23:263-292. doi: 10.1146/annurev.cellbio.22.010605.093554.
Hoe, H-S, Tran, TS, Matsuoka, Y, Howell, BW and Rebeck, GW. (2006). DAB1 and Reelin effects on amyloid precursor protein and ApoE receptor 2 trafficking and processing. J. Biol. Chem. 281:35176-35185. doi: 10.1074/jbc.M602162200.
Huber, AB, Kania, A, Tran, TS, Gu, C, De Marco Garcia, N, Lieberam, I, Johnson, D, Jessell, TM, Ginty, DD and Kolodkin, AL. (2005). Distinct roles for secreted semaphorin signaling in spinal motor axon guidance. Neuron, 48:949-964. doi: 10.106/j.neuron.2005.12.003.
Chen, K, Ochalski, PG, Tran, TS, Sahir, N, Schubert, M, Pramatarova, A and Howell, BW. (2004). Interaction between Dab1 and CrkII is promoted by Reelin signaling. J. Cell Sci. 117:4527-36.
Tran, TS and Phelps, PE. (2004). Embryonic GABAergic spinal commissural neurons project rostrally to mesencephalic targets. J. Comp. Neurol. 475:327-339.
Tran, TS, Alijani, A and Phelps, PE. (2003). Unique developmental patterns of GABAergic neurons in rat spinal cord. J. Comp. Neurol. 456:112-126.
Tran, TS and Phelps, PE. (2000). Axons crossing in the ventral commissure express L1 and GAD65 in developing rat spinal cord. Dev. Neurosci. 22:228-236.
Phelps, PE, Alijani, A and Tran, TS. (1999). Ventrally located commissural neurons express the GABAergic phenotype in developing rat spinal cord. J. Comp. Neurol. 409:285-298.
Faculty advisor for majors in:
Clinical Laboratory Sciences
Medical Imaging Sciences